› 3al4

X-ray diffraction
2.87Å resolution

Crystal structure of the swine-origin A (H1N1)-2009 influenza A virus hemagglutinin (HA) reveals similar antigenicity to that of the 1918 pandemic virus

Released:
DOI: 10.2210/pdb3al4/pdb
PDB entry 3al4 coloured by chain, front view.
PDB entry 3al4 coloured by chain, side view.
PDB entry 3al4 coloured by chain, top view.
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Primary publication:
Crystal structure of the swine-origin A (H1N1)-2009 influenza A virus hemagglutinin (HA) reveals similar antigenicity to that of the 1918 pandemic virus.
Zhang W, Qi J, Shi Y, Li Q, Gao F, Sun Y, Lu X, Lu Q, Vavricka CJ, Liu D, Yan J, Gao GF
Protein Cell 1 459-67 (2010)
PMID: 21203961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111238 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 333 amino acids
Theoretical weight: 36.99 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: C3W5S1 (Residues: 18-344; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 181 amino acids
Theoretical weight: 20.76 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: C3W5S1 (Residues: 345-520; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 16 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 66.02Å b: 115.19Å c: 114.985Å
α: 62.31° β: 77.94° γ: 81.05°
R-values:
R R work R free
0.247 0.246 0.27
Expression system: Spodoptera frugiperda

Quick links

Citations

6 review citations

Molecular basis of the structure and function of H1 hemagglutinin of influenza virus.
Sriwilaijaroen et al. (2012)
5 more

24 mentions without citation

A recommended numbering scheme for influenza A HA subtypes.
Burke et al. (2014)
23 more