› 3al7

X-ray diffraction
1.1Å resolution

Recombinant thaumatin I at 1.1 A

Released:
DOI: 10.2210/pdb3al7/pdb
PDB entry 3al7 coloured by chain, front view.
PDB entry 3al7 coloured by chain, side view.
PDB entry 3al7 coloured by chain, top view.
Source organism: Thaumatococcus daniellii
Primary publication:
High-resolution structure of the recombinant sweet-tasting protein thaumatin I.
Masuda T, Ohta K, Mikami B, Kitabatake N
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 652-8 (2011)
PMID: 21636903

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thaumatin I Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 22.23 KDa
Source organism: Thaumatococcus daniellii
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P02883 (Residues: 23-229; Coverage: 97%)
Sequence domains: Thaumatin family
Structure domains: Thaumatin

Ligands and Environments

2 bound ligands:
Ligand TLA 2 x TLA
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P41212
Unit cell:
a: 57.699Å b: 57.699Å c: 149.776Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.108 not available 0.135
Expression system: Komagataella pastoris

Quick links

Citations

6 citation in other articles

Grease matrix as a versatile carrier of proteins for serial crystallography.
Sugahara et al. (2015)
5 more

4 mentions without citation

A Hypersweet Protein: Removal of The Specific Negative Charge at Asp21 Enhances Thaumatin Sweetness.
Masuda et al. (2016)
3 more