› 3ao9

X-ray diffraction
2.1Å resolution

Crystal structure of the C-terminal domain of sequence-specific ribonuclease

Released:
DOI: 10.2210/pdb3ao9/pdb
PDB entry 3ao9 coloured by chain, front view.
PDB entry 3ao9 coloured by chain, side view.
PDB entry 3ao9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Identification of the catalytic residues of sequence-specific and histidine-free ribonuclease colicin E5.
Inoue-Ito S, Yajima S, Fushinobu S, Nakamura S, Ogawa T, Hidaka M, Masaki H
J Biochem 152 365-72 (2012)
PMID: 22815490

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148241 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-E5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 12.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P18000 (Residues: 66-180; Coverage: 64%)
Gene name: col
Sequence domains: Colicin E5 ribonuclease domain
Structure domains: YaeB-like fold

Ligands and Environments

1 bound ligand:
Ligand CD 4 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P3121
Unit cell:
a: 62.71Å b: 62.71Å c: 100.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.222 0.259
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Generation of 2',3'-Cyclic Phosphate-Containing RNAs as a Hidden Layer of the Transcriptome.
Shigematsu et al. (2018)
1 more