› 3ap1

X-ray diffraction
1.9Å resolution

Crystal structure of human tyrosylprotein sulfotransferase-2 complexed with PAP and C4 peptide

Released:
DOI: 10.2210/pdb3ap1/pdb
PDB entry 3ap1 coloured by chain, front view.
PDB entry 3ap1 coloured by chain, side view.
PDB entry 3ap1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction.
Teramoto T, Fujikawa Y, Kawaguchi Y, Kurogi K, Soejima M, Adachi R, Nakanishi Y, Mishiro-Sato E, Liu MC, Sakakibara Y, Suiko M, Kimura M, Kakuta Y
Nat Commun 4 1572 (2013)
PMID: 23481380

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130075 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein-tyrosine sulfotransferase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 37.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60704 (Residues: 43-359; Coverage: 84%)
Gene name: TPST2
Sequence domains: Sulfotransferase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
C4 peptide Chains: S, T
Molecule details ›
Chains: S, T
Length: 9 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand A3P 2 x A3P
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: R32
Unit cell:
a: 138.263Å b: 138.263Å c: 227.994Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.222
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

The Regulation of Steroid Action by Sulfation and Desulfation.
Mueller et al. (2015)
6 more

12 mentions without citation

Pharmacogenetics of drug-metabolizing enzymes: implications for a safer and more effective drug therapy.
Ingelman-Sundberg et al. (2005)
11 more