› 3ar5

X-ray diffraction
2.2Å resolution

Calcium pump crystal structure with bound TNP-AMP and TG

Released:
DOI: 10.2210/pdb3ar5/pdb
PDB entry 3ar5 coloured by chain, front view.
PDB entry 3ar5 coloured by chain, side view.
PDB entry 3ar5 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Trinitrophenyl derivatives bind differently from parent adenine nucleotides to Ca2+-ATPase in the absence of Ca2+.
Toyoshima C, Yonekura S, Tsueda J, Iwasawa S
Proc Natl Acad Sci U S A 108 1833-8 (2011)
PMID: 21239683

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand TG1 1 x TG1
Ligand TM1 1 x TM1
Ligand PTY 3 x PTY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P41212
Unit cell:
a: 71.683Å b: 71.683Å c: 589.075Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.26 0.288

Quick links

Citations

6 review citations

Ion pathways in the sarcoplasmic reticulum Ca2+-ATPase.
Bublitz et al. (2013)
5 more

2 mentions without citation

Trinitrophenyl derivatives bind differently from parent adenine nucleotides to Ca2+-ATPase in the absence of Ca2+.
Toyoshima et al. (2011)
1 more