› 3atg

X-ray diffraction
1.66Å resolution

endo-1,3-beta-glucanase from Cellulosimicrobium cellulans

Released:
DOI: 10.2210/pdb3atg/pdb
PDB entry 3atg coloured by chain, front view.
PDB entry 3atg coloured by chain, side view.
PDB entry 3atg coloured by chain, top view.
Source organism: Cellulosimicrobium cellulans
Primary publication:
Structural and thermodynamic characterization of endo-1,3-β-glucanase: Insights into the substrate recognition mechanism.
Oda M, Inaba S, Kamiya N, Bekker GJ, Mikami B
Biochim Biophys Acta Proteins Proteom 1866 415-425 (2018)
PMID: 29246508

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH16 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 27.71 KDa
Source organism: Cellulosimicrobium cellulans
UniProt:
  • Canonical: G1ED17 (Residues: 53-308; Coverage: 65%)
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand PO4 5 x PO4
Ligand GOL 3 x GOL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P43
Unit cell:
a: 71.309Å b: 71.309Å c: 60.069Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.166

Quick links

Citations

2 review citations

The Biological Structure Model Archive (BSM-Arc): an archive for in silico models and simulations.
Bekker et al. (2020)
1 more

3 mentions without citation

The β-glucanase ZgLamA from Zobellia galactanivorans evolved a bent active site adapted for efficient degradation of algal laminarin.
Labourel et al. (2014)
2 more