› 3auk

X-ray diffraction
1.66Å resolution

Crystal structure of a lipase from Geobacillus sp. SBS-4S

Released:
DOI: 10.2210/pdb3auk/pdb
PDB entry 3auk coloured by chain, front view.
PDB entry 3auk coloured by chain, side view.
PDB entry 3auk coloured by chain, top view.
Source organism: Geobacillus sp. SBS-4S
Entry authors: Angkawidjaja C, Tayyab M, Rashid N, Kanaya S

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 43.34 KDa
Source organism: Geobacillus sp. SBS-4S
Expression system: Escherichia coli
UniProt:
  • Canonical: B9ZZP0 (Residues: 1-389; Coverage: 100%)
Gene name: Lip
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 1 x CA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 55.131Å b: 71.745Å c: 126.262Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.19
Expression system: Escherichia coli

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