› 3auw

X-ray diffraction
3.56Å resolution

Cytoplasmic domain of inward rectifier potassium channel Kir3.2 in complex with cadmium

Released:
DOI: 10.2210/pdb3auw/pdb
PDB entry 3auw coloured by chain, front view.
PDB entry 3auw coloured by chain, side view.
PDB entry 3auw coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Inverse agonist-like action of cadmium on G-protein-gated inward-rectifier K(+) channels.
Inanobe A, Matsuura T, Nakagawa A, Kurachi Y
Biochem Biophys Res Commun 407 366-71 (2011)
PMID: 21396912

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155751 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
G protein-activated inward rectifier potassium channel 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 26 amino acids
Theoretical weight: 3.12 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P48542 (Residues: 53-74; Coverage: 5%)
Gene names: Girk2, Kcnj6, Kcnj7, W
G protein-activated inward rectifier potassium channel 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 182 amino acids
Theoretical weight: 20.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P48542 (Residues: 200-381; Coverage: 43%)
Gene names: Girk2, Kcnj6, Kcnj7, W
Sequence domains: Inward rectifier potassium channel C-terminal domain
Structure domains: G protein-activated inward rectifier potassium channel 1

Ligands and Environments

3 bound ligands:
Ligand CD 2 x CD
Ligand MG 2 x MG
Ligand EOH 2 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P4212
Unit cell:
a: 100.808Å b: 100.808Å c: 105.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.286 0.281 0.331
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Membrane channels as integrators of G-protein-mediated signaling.
Inanobe et al. (2014)
7 other articles

2 mentions without citation

L-DOPA-quinone Mediated Recovery from GIRK Channel Firing Inhibition in Dopaminergic Neurons.
Bizzarri et al. (2019)
1 more