› 3awk

X-ray diffraction
2Å resolution

Crystal structure of the polyketide synthase 1 from huperzia serrata

Released:
DOI: 10.2210/pdb3awk/pdb
PDB entry 3awk coloured by chain, front view.
PDB entry 3awk coloured by chain, side view.
PDB entry 3awk coloured by chain, top view.
Source organism: Huperzia serrata
Primary publication:
Synthesis of unnatural alkaloid scaffolds by exploiting plant polyketide synthase.
Morita H, Yamashita M, Shi SP, Wakimoto T, Kondo S, Kato R, Sugio S, Kohno T, Abe I
Proc Natl Acad Sci U S A 108 13504-9 (2011)
PMID: 21825160

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase-like polyketide synthase Chain: A
Molecule details ›
Chain: A
Length: 402 amino acids
Theoretical weight: 43.66 KDa
Source organism: Huperzia serrata
Expression system: Escherichia coli
UniProt:
  • Canonical: A3E7Z7 (Residues: 1-399; Coverage: 100%)
Gene name: Pks1
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU
Spacegroup: I212121
Unit cell:
a: 73.3Å b: 85Å c: 137.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.234
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The Origin and Evolution of Plant Flavonoid Metabolism.
Yonekura-Sakakibara et al. (2019)
11 more

3 mentions without citation

How structural subtleties lead to molecular diversity for the type III polyketide synthases.
Morita et al. (2019)
2 more