3ax5

X-ray diffraction
2.2Å resolution

Crystal structure of rat TOM20-ALDH presequence complex: A complex (form1) between Tom20 and a disulfide-bridged presequence peptide containing D-Cys and L-Cys at the i and i+3 positions.

Released:

Function and Biology Details

Reaction catalysed:
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
  • not assigned
Biological process:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-145970 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial import receptor subunit TOM20 homolog Chains: A, C
Molecule details ›
Chains: A, C
Length: 73 amino acids
Theoretical weight: 8.02 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62760 (Residues: 59-126; Coverage: 47%)
Gene name: Tomm20
Sequence domains: MAS20 protein import receptor
Structure domains: Mitochondrial outer membrane translocase complex, subunit Tom20 domain
Aldehyde dehydrogenase, mitochondrial Chains: B, D
Molecule details ›
Chains: B, D
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P11884 (Residues: 12-20; Coverage: 2%)
Gene name: Aldh2

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I222
Unit cell:
a: 41.745Å b: 77.401Å c: 116.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.276
Expression systems:
  • Escherichia coli
  • Not provided