› 3ays

X-ray diffraction
2.2Å resolution

GH5 endoglucanase from a ruminal fungus in complex with cellotriose

Released:
DOI: 10.2210/pdb3ays/pdb
PDB entry 3ays coloured by chain, front view.
PDB entry 3ays coloured by chain, side view.
PDB entry 3ays coloured by chain, top view.
Source organism: Piromyces rhizinflatus
Primary publication:
Substrate binding of a GH5 endoglucanase from the ruminal fungus Piromyces rhizinflata.
Tseng CW, Ko TP, Guo RT, Huang JW, Wang HC, Huang CH, Cheng YS, Wang AH, Liu JR
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1189-94 (2011)
PMID: 22102024

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-194082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycoside hydrolase family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 43.32 KDa
Source organism: Piromyces rhizinflatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9URH5 (Residues: 1-362; Coverage: 100%)
Gene name: eglA
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P6122
Unit cell:
a: 82.204Å b: 82.204Å c: 221.856Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.162 0.218
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Anaerobic gut fungi: Advances in isolation, culture, and cellulolytic enzyme discovery for biofuel production.
Haitjema et al. (2014)
1 more

5 mentions without citation

Family 46 Carbohydrate-binding Modules Contribute to the Enzymatic Hydrolysis of Xyloglucan and β-1,3-1,4-Glucans through Distinct Mechanisms.
Venditto et al. (2015)
4 more