› 3b0f

X-ray diffraction
1.4Å resolution

Crystal structure of the UBA domain of p62 and its interaction with ubiquitin

Released:
DOI: 10.2210/pdb3b0f/pdb
PDB entry 3b0f coloured by chain, front view.
PDB entry 3b0f coloured by chain, side view.
PDB entry 3b0f coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the ubiquitin-associated (UBA) domain of p62 and its interaction with ubiquitin.
Isogai S, Morimoto D, Arita K, Unzai S, Tenno T, Hasegawa J, Sou YS, Komatsu M, Tanaka K, Shirakawa M, Tochio H
J Biol Chem 286 31864-74 (2011)
PMID: 21715324

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sequestosome-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 53 amino acids
Theoretical weight: 5.82 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q64337 (Residues: 391-438; Coverage: 11%)
Gene names: A170, STAP, Sqstm1
Sequence domains: UBA domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P43212
Unit cell:
a: 72.967Å b: 72.967Å c: 32.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.201
Expression system: Escherichia coli

Quick links

Citations

23 review citations

p62/SQSTM1 functions as a signaling hub and an autophagy adaptor.
Katsuragi et al. (2015)
22 more

10 mentions without citation

Structure biology of selective autophagy receptors.
Kim et al. (2016)
9 more