› 3b1d

X-ray diffraction
1.66Å resolution

Crystal structure of betaC-S lyase from Streptococcus anginosus in complex with L-serine: External aldimine form

Released:
DOI: 10.2210/pdb3b1d/pdb
PDB entry 3b1d coloured by chain, front view.
PDB entry 3b1d coloured by chain, side view.
PDB entry 3b1d coloured by chain, top view.
Source organism: Streptococcus anginosus
Primary publication:
Structural insights into catalysis by βC-S lyase from Streptococcus anginosus.
Kezuka Y, Yoshida Y, Nonaka T
Proteins 80 2447-58 (2012)
PMID: 22674431

Function and Biology Details

Reaction catalysed: 
(1a) an L-cysteine-S-conjugate = a thiol + 2-aminoporp-2-enoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cysteine-S-conjugate beta-lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 392 amino acids
Theoretical weight: 44.65 KDa
Source organism: Streptococcus anginosus
Expression system: Escherichia coli
UniProt:
  • Canonical: A6BMJ3 (Residues: 2-388; Coverage: 100%)
Gene name: lcd
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
3 bound ligands:
Ligand PLS 4 x PLS
Ligand EPE 11 x EPE
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 66.965Å b: 111.302Å c: 217.193Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.177
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Characterization of C-S Lyase from C. diphtheriae: a possible target for new antimicrobial drugs.
Astegno et al. (2013)
5 more