› 3b21

X-ray diffraction
2.01Å resolution

Crystal structure of OspI from Shigella flexineri

Released:
DOI: 10.2210/pdb3b21/pdb
PDB entry 3b21 coloured by chain, front view.
PDB entry 3b21 coloured by chain, side view.
PDB entry 3b21 coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
The Shigella flexneri effector OspI deamidates UBC13 to dampen the inflammatory response.
Sanada T, Kim M, Mimuro H, Suzuki M, Ogawa M, Oyama A, Ashida H, Kobayashi T, Koyama T, Nagai S, Shibata Y, Gohda J, Inoue J, Mizushima T, Sasakawa C
Nature 483 623-6 (2012)
PMID: 22407319

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORF169b Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 24.15 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VSD5 (Residues: 1-212; Coverage: 100%)
Gene names: ORF169b, SF_p0209
Sequence domains: Shigella glutamine deamidase OspI
Structure domains: Cathepsin B; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2221
Unit cell:
a: 67.699Å b: 91.237Å c: 67.862Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.226
Expression system: Escherichia coli

Quick links

Citations

51 review citations

Recent advances in understanding enteric pathogenic Escherichia coli.
Croxen et al. (2013)
50 more

5 mentions without citation

What a difference a Dalton makes: bacterial virulence factors modulate eukaryotic host cell signaling systems via deamidation.
Washington et al. (2013)
4 more