› 3b33

X-ray diffraction
1.83Å resolution

Crystal structure of the PAS domain of nitrogen regulation protein NR(II) from Vibrio parahaemolyticus

Released:
DOI: 10.2210/pdb3b33/pdb
PDB entry 3b33 coloured by chain, front view.
PDB entry 3b33 coloured by chain, side view.
PDB entry 3b33 coloured by chain, top view.
Source organism: Vibrio parahaemolyticus RIMD 2210633
Entry authors: Osipiuk J, Sather A, Abdullah J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensory histidine kinase/phosphatase NtrB Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.85 KDa
Source organism: Vibrio parahaemolyticus RIMD 2210633
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q87TF0 (Residues: 1-112; Coverage: 32%)
Gene name: VP0119
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6322
Unit cell:
a: 81.115Å b: 81.115Å c: 67.467Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.184 0.196
Expression system: Escherichia coli BL21(DE3)

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