› 3b46

X-ray diffraction
2Å resolution

Crystal Structure of Bna3p, a Putative Kynurenine Aminotransferase from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3b46/pdb
PDB entry 3b46 coloured by chain, front view.
PDB entry 3b46 coloured by chain, side view.
PDB entry 3b46 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Identification of formyl kynurenine formamidase and kynurenine aminotransferase from Saccharomyces cerevisiae using crystallographic, bioinformatic and biochemical evidence.
Wogulis M, Chew ER, Donohoue PD, Wilson DK
Biochemistry 47 1608-21 (2008)
PMID: 18205391

Function and Biology Details

Reaction catalysed: 
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable kynurenine--oxoglutarate transaminase BNA3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 447 amino acids
Theoretical weight: 50.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47039 (Residues: 1-444; Coverage: 100%)
Gene names: BNA3, J1138, YJL060W
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P21
Unit cell:
a: 55.78Å b: 66.52Å c: 115.2Å
α: 90° β: 90.75° γ: 90°
R-values:
R R work R free
0.227 0.227 0.271
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Structure, expression, and function of kynurenine aminotransferases in human and rodent brains.
Han et al. (2010)
6 more

2 mentions without citation

Global DNA methylation profiling reveals silencing of a secreted form of Epha7 in mouse and human germinal center B-cell lymphomas.
Dawson et al. (2007)
1 more