› 3b59

X-ray diffraction
2.53Å resolution

Crystal structure of the Mn(II)-bound glyoxalase from Novosphingobium aromaticivorans

Released:
DOI: 10.2210/pdb3b59/pdb
PDB entry 3b59 coloured by chain, front view.
PDB entry 3b59 coloured by chain, side view.
PDB entry 3b59 coloured by chain, top view.
Source organism: Novosphingobium aromaticivorans DSM 12444
Entry authors: Madegowda M, Eswaramoorthy S, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VOC domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 310 amino acids
Theoretical weight: 34.57 KDa
Source organism: Novosphingobium aromaticivorans DSM 12444
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2GAG3 (Residues: 2-300; Coverage: 100%)
Gene name: Saro_0713
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

1 bound ligand:
Ligand MN 6 x MN
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I222
Unit cell:
a: 144.997Å b: 168.348Å c: 202.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.257
Expression system: Escherichia coli BL21(DE3)

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