› 3b5g

X-ray diffraction
1.9Å resolution

Crystal Structure of the Unstable and Highly Fibrillogenic PRO7SER Mutant of the Recombinant Variable Domain 6AJL2

Released:
DOI: 10.2210/pdb3b5g/pdb
PDB entry 3b5g coloured by chain, front view.
PDB entry 3b5g coloured by chain, side view.
PDB entry 3b5g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A single mutation at the sheet switch region results in conformational changes favoring lambda6 light-chain fibrillogenesis.
Hernández-Santoyo A, del Pozo Yauner L, Fuentes-Silva D, Ortiz E, Rudiño-Piñera E, Sánchez-López R, Horjales E, Becerril B, Rodríguez-Romero A
J Mol Biol 396 280-92 (2010)
PMID: 19941869

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AMYLOID LAMBDA 6 LIGHT CHAIN VARIABLE REGION PIP Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 11.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand ACT 2 x ACT
Ligand GOL 7 x GOL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 40.77Å b: 68.59Å c: 83.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.164 0.21
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Amyloid formation in light chain amyloidosis.
Ramirez-Alvarado M. (2012)
4 more

4 mentions without citation

Systemic amyloidoses.
Blancas-Mejía et al. (2013)
3 more