› 3b5n

X-ray diffraction
1.6Å resolution

Structure of the yeast plasma membrane SNARE complex

Released:
DOI: 10.2210/pdb3b5n/pdb
PDB entry 3b5n coloured by chain, front view.
PDB entry 3b5n coloured by chain, side view.
PDB entry 3b5n coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The structure of the yeast plasma membrane SNARE complex reveals destabilizing water-filled cavities.
Strop P, Kaiser SE, Vrljic M, Brunger AT
J Biol Chem 283 1113-9 (2008)
PMID: 17956869

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151908 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Synaptobrevin homolog 1 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 61 amino acids
Theoretical weight: 6.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31109 (Residues: 27-86; Coverage: 51%)
Gene names: SNC1, YAL030W
Sequence domains: Synaptobrevin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Protein SSO1 Chains: B, F, J
Molecule details ›
Chains: B, F, J
Length: 69 amino acids
Theoretical weight: 7.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32867 (Residues: 189-257; Coverage: 24%)
Gene names: P1405, SSO1, YPL232W
Sequence domains: SNARE domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Protein transport protein SEC9 Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 70 amino acids
Theoretical weight: 7.69 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40357 (Residues: 433-499; Coverage: 10%)
Gene names: HSS7, SEC9, YGR009C
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Protein transport protein SEC9 Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 64 amino acids
Theoretical weight: 7.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40357 (Residues: 589-650; Coverage: 10%)
Gene names: HSS7, SEC9, YGR009C
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 76.956Å b: 48.113Å c: 110.265Å
α: 90° β: 97.77° γ: 90°
R-values:
R R work R free
0.206 0.206 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Single-molecule studies of the neuronal SNARE fusion machinery.
Brunger et al. (2009)
3 more

5 mentions without citation

RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution.
Venkataraman et al. (2018)
4 more