› 3b5q

X-ray diffraction
2.4Å resolution

Crystal structure of a putative sulfatase (NP_810509.1) from Bacteroides thetaiotaomicron VPI-5482 at 2.40 A resolution

Released:
DOI: 10.2210/pdb3b5q/pdb
PDB entry 3b5q coloured by chain, front view.
PDB entry 3b5q coloured by chain, side view.
PDB entry 3b5q coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183736 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta 4,5-hexuronate-2-O-sulfatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 482 amino acids
Theoretical weight: 54.34 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A7C8 (Residues: 1-481; Coverage: 100%)
Gene name: BT_1596
Sequence domains: Sulfatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand EDO 25 x EDO
Ligand PEG 3 x PEG
Ligand EPE 3 x EPE
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P41
Unit cell:
a: 106.275Å b: 106.275Å c: 111.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.149 0.206
Expression system: Escherichia coli

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