› 3b7f

X-ray diffraction
2.2Å resolution

Crystal structure of a putative glycosyl hydrolase with bnr repeats (reut_b4987) from ralstonia eutropha jmp134 at 2.20 A resolution

Released:
DOI: 10.2210/pdb3b7f/pdb
PDB entry 3b7f coloured by chain, front view.
PDB entry 3b7f coloured by chain, side view.
PDB entry 3b7f coloured by chain, top view.
Source organism: Cupriavidus pinatubonensis JMP134
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase, BNR repeat protein Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 43.85 KDa
Source organism: Cupriavidus pinatubonensis JMP134
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46R99 (Residues: 1-393; Coverage: 100%)
Gene name: Reut_B4987
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand EDO 17 x EDO
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I4122
Unit cell:
a: 114.31Å b: 114.31Å c: 161.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.216
Expression system: Escherichia coli

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