› 3b89

X-ray diffraction
2.6Å resolution

Crystal structure of rRNA methylase from Escherichia coli

Released:
DOI: 10.2210/pdb3b89/pdb
PDB entry 3b89 coloured by chain, front view.
PDB entry 3b89 coloured by chain, side view.
PDB entry 3b89 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Eswaramoorthy S, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(1405) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(1405) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
16S rRNA (guanine(1405)-N(7))-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 27.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q763K9 (Residues: 2-251; Coverage: 100%)
Gene names: AKG29_00615, BANRA_05536, E4K51_26945, IPF_158, RmtB, pHN3A11_015, pHN7A8_021, pHNFP460_047, rmtB
Sequence domains: Ribosomal RNA methyltransferase (FmrO)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 5GP 1 x 5GP
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I222
Unit cell:
a: 56.262Å b: 94.131Å c: 96.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.226 0.282
Expression system: Escherichia coli

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