› 3b8i

X-ray diffraction
1.9Å resolution

Crystal Structure of Oxaloacetate Decarboxylase from Pseudomonas Aeruginosa (PA4872) in complex with oxalate and Mg2+.

Released:
DOI: 10.2210/pdb3b8i/pdb
PDB entry 3b8i coloured by chain, front view.
PDB entry 3b8i coloured by chain, side view.
PDB entry 3b8i coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure and function of PA4872 from Pseudomonas aeruginosa, a novel class of oxaloacetate decarboxylase from the PEP mutase/isocitrate lyase superfamily.
Narayanan BC, Niu W, Han Y, Zou J, Mariano PS, Dunaway-Mariano D, Herzberg O
Biochemistry 47 167-82 (2008)
PMID: 18081320

Function and Biology Details

Reaction catalysed: 
Oxaloacetate = pyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxaloacetate decarboxylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 287 amino acids
Theoretical weight: 31.36 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HUU1 (Residues: 1-287; Coverage: 100%)
Gene name: PA4872
Sequence domains: Phosphoenolpyruvate phosphomutase
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

3 bound ligands:
Ligand MG 6 x MG
Ligand OXL 6 x OXL
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 260.065Å b: 83.835Å c: 104.874Å
α: 90° β: 112.14° γ: 90°
R-values:
R R work R free
0.197 0.191 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Functionally diverse biotin-dependent enzymes with oxaloacetate decarboxylase activity.
Lietzan et al. (2014)
11 other articles

2 mentions without citation

Structure and function of PA4872 from Pseudomonas aeruginosa, a novel class of oxaloacetate decarboxylase from the PEP mutase/isocitrate lyase superfamily.
Narayanan et al. (2008)
1 more