› 3b8p

X-ray diffraction
3.1Å resolution

Fragment of WzzB, Polysaccharide Co-polymerase from Salmonella Typhimurium

Released:
DOI: 10.2210/pdb3b8p/pdb
PDB entry 3b8p coloured by chain, front view.
PDB entry 3b8p coloured by chain, side view.
PDB entry 3b8p coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Bacterial polysaccharide co-polymerases share a common framework for control of polymer length.
Tocilj A, Munger C, Proteau A, Morona R, Purins L, Ajamian E, Wagner J, Papadopoulos M, Van Den Bosch L, Rubinstein JL, Féthière J, Matte A, Cygler M
Nat Struct Mol Biol 15 130-8 (2008)
PMID: 18204465

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
homo pentamer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chain length determinant protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 243 amino acids
Theoretical weight: 27.46 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04866 (Residues: 54-294; Coverage: 74%)
Gene names: STM2079, cld, rol, wzzB
Sequence domains: Chain length determinant protein
Structure domains: FepE-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 87.972Å b: 112.744Å c: 170.929Å
α: 90° β: 97.34° γ: 90°
R-values:
R R work R free
0.257 0.255 0.301
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Pivotal roles of the outer membrane polysaccharide export and polysaccharide copolymerase protein families in export of extracellular polysaccharides in gram-negative bacteria.
Cuthbertson et al. (2009)
17 more

4 mentions without citation

Mutagenesis and chemical cross-linking suggest that Wzz dimer stability and oligomerization affect lipopolysaccharide O-antigen modal chain length control.
Papadopoulos et al. (2010)
3 more