› 3b96

X-ray diffraction
1.91Å resolution

Structural Basis for Substrate Fatty-Acyl Chain Specificity: Crystal Structure of Human Very-Long-Chain Acyl-CoA Dehydrogenase

Released:
DOI: 10.2210/pdb3b96/pdb
PDB entry 3b96 coloured by chain, front view.
PDB entry 3b96 coloured by chain, side view.
PDB entry 3b96 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for substrate fatty acyl chain specificity: crystal structure of human very-long-chain acyl-CoA dehydrogenase.
McAndrew RP, Wang Y, Mohsen AW, He M, Vockley J, Kim JJ
J Biol Chem 283 9435-43 (2008)
PMID: 18227065

Function and Biology Details

Reaction catalysed: 
A very-long-chain acyl-CoA + electron-transfer flavoprotein = a very-long-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 587 amino acids
Theoretical weight: 63.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49748 (Residues: 69-655; Coverage: 90%)
Gene names: ACADVL, VLCAD
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand MYA 1 x MYA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: null, APS BEAMLINE 19-ID, APS BEAMLINE 5ID-B
Spacegroup: C2221
Unit cell:
a: 74.616Å b: 107.879Å c: 149.776Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.159 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Dynamics of Human Mitochondrial Complex I Assembly: Implications for Neurodegenerative Diseases.
Giachin et al. (2016)
4 more

18 mentions without citation

Structural basis for substrate fatty acyl chain specificity: crystal structure of human very-long-chain acyl-CoA dehydrogenase.
McAndrew et al. (2008)
17 more