› 3b9p

X-ray diffraction
2.7Å resolution

Spastin

Released:
DOI: 10.2210/pdb3b9p/pdb
PDB entry 3b9p coloured by chain, front view.
PDB entry 3b9p coloured by chain, side view.
PDB entry 3b9p coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin.
Roll-Mecak A, Vale RD
Nature 451 363-7 (2008)
PMID: 18202664

Function and Biology Details

Reaction catalysed: 
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spastin Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 32.85 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I0P1 (Residues: 463-758; Coverage: 39%)
Gene names: CG5977, spas
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P65
Unit cell:
a: 83.901Å b: 83.901Å c: 95.014Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.245 0.287
Expression system: Escherichia coli

Quick links

Citations

50 review citations

Axonal transport deficits and neurodegenerative diseases.
Millecamps et al. (2013)
49 more

8 mentions without citation

Microtubule-severing enzymes: From cellular functions to molecular mechanism.
McNally et al. (2018)
7 more