› 3b9w

X-ray diffraction
1.3Å resolution

The 1.3 A resolution structure of Nitrosomonas europaea Rh50 and mechanistic implications for NH3 transport by Rhesus family proteins

Released:
DOI: 10.2210/pdb3b9w/pdb
PDB entry 3b9w coloured by chain, front view.
PDB entry 3b9w coloured by chain, side view.
PDB entry 3b9w coloured by chain, top view.
Source organism: Nitrosomonas europaea
Primary publication:
The 1.3-A resolution structure of Nitrosomonas europaea Rh50 and mechanistic implications for NH3 transport by Rhesus family proteins.
Lupo D, Li XD, Durand A, Tomizaki T, Cherif-Zahar B, Matassi G, Merrick M, Winkler FK
Proc Natl Acad Sci U S A 104 19303-8 (2007)
PMID: 18032606

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter AmtB-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 407 amino acids
Theoretical weight: 42.78 KDa
Source organism: Nitrosomonas europaea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q82X47 (Residues: 25-425; Coverage: 100%)
Gene names: NE0448, Rh50
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

2 bound ligands:
Ligand BOG 1 x BOG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R3
Unit cell:
a: 100.061Å b: 100.061Å c: 143.828Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.147 0.171
Expression system: Escherichia coli

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Citations

21 review citations

Ion regulation in fish gills: recent progress in the cellular and molecular mechanisms.
Hwang et al. (2011)
20 more

10 mentions without citation

Function of human Rh based on structure of RhCG at 2.1 A.
Gruswitz et al. (2010)
9 more