3baa

X-ray diffraction
1.9Å resolution

Structural Basis for the Inhibition of Bacterial NAD+ Dependent DNA Ligase

Released:
Source organism: Enterococcus faecalis
Entry author: Pinko C

Function and Biology Details

Reaction catalysed:
(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182853 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 38.03 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q837V6 (Residues: 1-324; Coverage: 48%)
Gene names: EF_0722, ligA
Sequence domains: NAD-dependent DNA ligase adenylation domain
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: C2
Unit cell:
a: 90.494Å b: 86.363Å c: 56.833Å
α: 90° β: 101.1° γ: 90°
R-values:
R R work R free
0.187 0.187 0.216
Expression system: Escherichia coli