› 3bae

X-ray diffraction
1.59Å resolution

Crystal structure of Fab WO2 bound to the N terminal domain of Amyloid beta peptide (1-28)

Released:
DOI: 10.2210/pdb3bae/pdb
PDB entry 3bae coloured by chain, front view.
PDB entry 3bae coloured by chain, side view.
PDB entry 3bae coloured by chain, top view.
Source organisms:
Primary publication:
Amyloid-beta-anti-amyloid-beta complex structure reveals an extended conformation in the immunodominant B-cell epitope.
Miles LA, Wun KS, Crespi GA, Fodero-Tavoletti MT, Galatis D, Bagley CJ, Beyreuther K, Masters CL, Cappai R, McKinstry WJ, Barnham KJ, Parker MW
J Mol Biol 377 181-92 (2008)
PMID: 18237744

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106870 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 24.14 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: A2NHM3 (Residues: 1-218; Coverage: 100%)
Gene names: Igk-C, Igkc
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 228 amino acids
Theoretical weight: 24.84 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q811U5 (Residues: 1-98, 99-118; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
P3(42) Chain: A
Molecule details ›
Chain: A
Length: 28 amino acids
Theoretical weight: 3.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 672-699; Coverage: 4%)
Gene names: A4, AD1, APP
Sequence domains: Beta-amyloid peptide (beta-APP)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 51.658Å b: 66.365Å c: 115.337Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.25
Expression system: Not provided

Quick links

Citations

2 review citations

Biotechnologically engineered protein binders for applications in amyloid diseases.
Haupt et al. (2014)
1 more

11 mentions without citation

Conformational selection in amyloid-based immunotherapy: Survey of crystal structures of antibody-amyloid complexes.
Ma et al. (2016)
10 more