› 3bap

X-ray diffraction
1.85Å resolution

Crystal Structure of the 25 kDa Subunit of Human Cleavage Factor Im

Released:
DOI: 10.2210/pdb3bap/pdb
PDB entry 3bap coloured by chain, front view.
PDB entry 3bap coloured by chain, side view.
PDB entry 3bap coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the 25 kDa subunit of human cleavage factor Im.
Coseno M, Martin G, Berger C, Gilmartin G, Keller W, Doublié S
Nucleic Acids Res 36 3474-83 (2008)
PMID: 18445629

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cleavage and polyadenylation specificity factor subunit 5 Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 26.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43809 (Residues: 1-227; Coverage: 100%)
Gene names: CFIM25, CPSF25, CPSF5, NUDT21
Sequence domains: Nucleotide hydrolase
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3121
Unit cell:
a: 80.115Å b: 80.115Å c: 72.209Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.244
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Pre-mRNA 3'-end processing complex assembly and function.
Chan et al. (2011)
6 more

6 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
5 more