› 3bb0

X-ray diffraction
1.5Å resolution

Crystal Structure of a Trapped Phosphate-Intermediate in Vanadium Apochloroperoxidase Catalyzing a Dephosphorylation Reaction

Released:
DOI: 10.2210/pdb3bb0/pdb
PDB entry 3bb0 coloured by chain, front view.
PDB entry 3bb0 coloured by chain, side view.
PDB entry 3bb0 coloured by chain, top view.
Source organism: Curvularia inaequalis
Primary publication:
Crystal structure of a trapped phosphate intermediate in vanadium apochloroperoxidase catalyzing a dephosphorylation reaction.
de Macedo-Ribeiro S, Renirie R, Wever R, Messerschmidt A
Biochemistry 47 929-34 (2008)
PMID: 18163651

Function and Biology Details

Reaction catalysed: 
RH + Cl(-) + H(2)O(2) = RCl + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155853 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vanadium chloroperoxidase Chain: A
Molecule details ›
Chain: A
Length: 609 amino acids
Theoretical weight: 67.67 KDa
Source organism: Curvularia inaequalis
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P49053 (Residues: 1-609; Coverage: 100%)
Gene name: CPO
Sequence domains: Vanadium chloroperoxidase N-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO3 1 x PO3
Ligand SO4 2 x SO4
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: R3
Unit cell:
a: 128.06Å b: 128.06Å c: 103.31Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.173 0.194
Expression system: Saccharomyces cerevisiae

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Citations

1 review citation

Exploring the chemistry and biology of vanadium-dependent haloperoxidases.
Winter et al. (2009)
3 other articles

2 mentions without citation

Chemical approaches to detect and analyze protein sulfenic acids.
Furdui et al. (2014)
1 more