› 3bbl

X-ray diffraction
2.35Å resolution

Crystal structure of a regulatory protein of LacI family from Chloroflexus aggregans

Released:
DOI: 10.2210/pdb3bbl/pdb
PDB entry 3bbl coloured by chain, front view.
PDB entry 3bbl coloured by chain, side view.
PDB entry 3bbl coloured by chain, top view.
Source organism: Chloroflexus aggregans DSM 9485
Entry authors: Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-110713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator, LacI family Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 32.7 KDa
Source organism: Chloroflexus aggregans DSM 9485
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8GD81 (Residues: 63-338; Coverage: 81%)
Gene name: Cagg_2268
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand EDO 4 x EDO
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6322
Unit cell:
a: 122.55Å b: 122.55Å c: 123.301Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.24 0.258
Expression system: Escherichia coli BL21(DE3)

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