› 3bbr

X-ray diffraction
2.25Å resolution

Crystal structure of the iGluR2 ligand binding core (S1S2J-N775S) in complex with a dimeric positive modulator as well as glutamate at 2.25 A resolution

Released:
DOI: 10.2210/pdb3bbr/pdb
PDB entry 3bbr coloured by chain, front view.
PDB entry 3bbr coloured by chain, side view.
PDB entry 3bbr coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural proof of a dimeric positive modulator bridging two identical AMPA receptor-binding sites.
Kaae BH, Harpsøe K, Kastrup JS, Sanz AC, Pickering DS, Metzler B, Clausen RP, Gajhede M, Sauerberg P, Liljefors T, Madsen U
Chem Biol 14 1294-303 (2007)
PMID: 18022568

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 29.19 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19491 (Residues: 413-796; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

5 bound ligands:
Ligand SO4 5 x SO4
Ligand CL 1 x CL
Ligand GLU 2 x GLU
Ligand BHY 1 x BHY
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P21212
Unit cell:
a: 99.08Å b: 121.78Å c: 47.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Functional insights from glutamate receptor ion channel structures.
Kumar et al. (2013)
6 more

5 mentions without citation

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.
Hansen et al. (2021)
4 more