› 3bbv

Electron Microscopy
10Å resolution

The tRNA(phe) fitted into the low resolution Cryo-EM map of the 50S.nc-tRNA.Hsp15 complex

Released:
DOI: 10.2210/pdb3bbv/pdb
PDB entry 3bbv coloured by chain, front view.
PDB entry 3bbv coloured by chain, side view.
PDB entry 3bbv coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Recycling of aborted ribosomal 50S subunit-nascent chain-tRNA complexes by the heat shock protein Hsp15.
Jiang L, Schaffitzel C, Bingel-Erlenmeyer R, Ban N, Korber P, Koning RI, de Geus DC, Plaisier JR, Abrahams JP
J Mol Biol 386 1357-67 (2009)
PMID: 19013177
Related structures: EMD-1455

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196633 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
tRNA(Phe) Chain: z
Molecule details ›
Chain: z
Length: 76 nucleotides
Theoretical weight: 24.65 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
Sequence domains: tRNA

Ligands and Environments

No bound ligands
6 modified residues:
Ligand 4SU 1 x 4SU
Ligand H2U 2 x H2U
Ligand PSU 3 x PSU
Ligand MIA 1 x MIA
Ligand 7MG 1 x 7MG
Ligand 5MU 1 x 5MU

Experiments and Validation Details

Entry percentile scores
Resolution: 10Å
Relevant EMDB volumes: EMD-1455
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The bacterial translation stress response.
Starosta et al. (2014)
7 more

1 mention without citation

Structure of Escherichia coli heat shock protein Hsp15 in complex with the ribosomal 50S subunit bearing peptidyl-tRNA.
Safdari et al. (2022)