› 3bcm

X-ray diffraction
2.25Å resolution

Crystal Structure of The Unswapped Form of P19A/L28Q/N67D BS-RNase

Released:
DOI: 10.2210/pdb3bcm/pdb
PDB entry 3bcm coloured by chain, front view.
PDB entry 3bcm coloured by chain, side view.
PDB entry 3bcm coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The buried diversity of bovine seminal ribonuclease: shape and cytotoxicity of the swapped non-covalent form of the enzyme.
Merlino A, Ercole C, Picone D, Pizzo E, Mazzarella L, Sica F
J Mol Biol 376 427-37 (2008)
PMID: 18164315

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seminal ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.62 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00669 (Residues: 27-150; Coverage: 100%)
Gene name: SRN
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 48.513Å b: 59.508Å c: 49.738Å
α: 90° β: 118.02° γ: 90°
R-values:
R R work R free
0.224 0.183 0.252
Expression system: Escherichia coli

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Citations

6 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
5 more

2 mentions without citation

Emerging biological functions of ribonuclease 1 and angiogenin.
Garnett et al. (2022)
1 more