› 3bcv

X-ray diffraction
2.35Å resolution

Crystal structure of a putative glycosyltransferase from Bacteroides fragilis

Released:
DOI: 10.2210/pdb3bcv/pdb
PDB entry 3bcv coloured by chain, front view.
PDB entry 3bcv coloured by chain, side view.
PDB entry 3bcv coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Entry authors: Palani K, Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyltransferase 2-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 27.54 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5LBM4 (Residues: 2-230; Coverage: 67%)
Gene name: BF9343_2714
Sequence domains: Glycosyl transferase family 2
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I23
Unit cell:
a: 152.434Å b: 152.434Å c: 152.434Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.257
Expression system: Escherichia coli BL21(DE3)

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