› 3bd9

X-ray diffraction
2.3Å resolution

human 3-O-sulfotransferase isoform 5 with bound PAP

Released:
DOI: 10.2210/pdb3bd9/pdb
PDB entry 3bd9 coloured by chain, front view.
PDB entry 3bd9 coloured by chain, side view.
PDB entry 3bd9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering sulfotransferases to modify heparan sulfate.
Xu D, Moon AF, Song D, Pedersen LC, Liu J
Nat Chem Biol 4 200-2 (2008)
PMID: 18223645

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heparan sulfate glucosamine 3-O-sulfotransferase 5 Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 32.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IZT8 (Residues: 88-346; Coverage: 75%)
Gene names: 3OST5, HS3OST5, HS3ST5
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand A3P 1 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 59.27Å b: 59.27Å c: 173.957Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.249
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Focus on antivirally active sulfated polysaccharides: from structure-activity analysis to clinical evaluation.
Ghosh et al. (2009)
9 more

6 mentions without citation

Heparan sulfate 3-O-sulfation: a rare modification in search of a function.
Thacker et al. (2014)
5 more