› 3bdf

X-ray diffraction
1.4Å resolution

Crystal structure of metal-free E. coli alkaline phosphatase (T155V)

Released:
DOI: 10.2210/pdb3bdf/pdb
PDB entry 3bdf coloured by chain, front view.
PDB entry 3bdf coloured by chain, side view.
PDB entry 3bdf coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Grigg JC, Murphy ME

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 458 amino acids
Theoretical weight: 48.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00634 (Residues: 22-471; Coverage: 100%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 55.503Å b: 103.634Å c: 88.55Å
α: 90° β: 105.8° γ: 90°
R-values:
R R work R free
0.163 0.162 0.184
Expression system: Escherichia coli BL21(DE3)

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