› 3bdn

X-ray diffraction
3.91Å resolution

Crystal Structure of the Lambda Repressor

Released:
DOI: 10.2210/pdb3bdn/pdb
PDB entry 3bdn coloured by chain, front view.
PDB entry 3bdn coloured by chain, side view.
PDB entry 3bdn coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
Crystal structure of the lambda repressor and a model for pairwise cooperative operator binding.
Stayrook S, Jaru-Ampornpan P, Ni J, Hochschild A, Lewis M
Nature 452 1022-5 (2008)
PMID: 18432246

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116089 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Repressor protein cI Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 26.05 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03034 (Residues: 2-237; Coverage: 100%)
Gene names: cI, lambdap88
Sequence domains:
Structure domains:
DNA (5'-D(*DAP*DAP*DTP*DAP*DCP*DCP*DAP*DCP*DTP*DGP*DGP*DCP*DGP*DGP*DTP*DGP*DAP*DTP*DAP*DT)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.16 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
DNA (5'-D(*DTP*DAP*DTP*DAP*DTP*DCP*DAP*DCP*DCP*DGP*DCP*DCP*DAP*DGP*DTP*DGP*DGP*DTP*DAP*DT)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C222
Unit cell:
a: 74.431Å b: 161.099Å c: 135.256Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.296 0.292 0.374
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Structural perspective of cooperative transcription factor binding.
Morgunova et al. (2017)
3 more

13 mentions without citation

Aspects of protein-DNA interactions: a review of quantitative thermodynamic theory for modelling synthetic circuits utilising LacI and CI repressors, IPTG and the reporter gene lacZ.
Munro et al. (2016)
12 more