› 3be6

X-ray diffraction
1.82Å resolution

Crystal structure of FitE (crystal form 2), a group III periplasmic siderophore binding protein

Released:
DOI: 10.2210/pdb3be6/pdb
PDB entry 3be6 coloured by chain, front view.
PDB entry 3be6 coloured by chain, side view.
PDB entry 3be6 coloured by chain, top view.
Source organism: Escherichia coli O157:H7 str. EDL933
Primary publication:
Trapping open and closed forms of FitE: a group III periplasmic binding protein.
Shi R, Proteau A, Wagner J, Cui Q, Purisima EO, Matte A, Cygler M
Proteins 75 598-609 (2009)
PMID: 19004000

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferrichrome-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 297 amino acids
Theoretical weight: 32.66 KDa
Source organism: Escherichia coli O157:H7 str. EDL933
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8XBR1 (Residues: 19-315; Coverage: 94%)
Gene names: ECs_3913, fitE
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

3 bound ligands:
Ligand CL 7 x CL
Ligand MG 1 x MG
Ligand GOL 3 x GOL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 50.815Å b: 109.12Å c: 221.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.219
Expression system: Escherichia coli

Quick links

Citations

4 review citations

A structural classification of substrate-binding proteins.
Berntsson et al. (2010)
3 more

4 mentions without citation

Specificity of Staphyloferrin B recognition by the SirA receptor from Staphylococcus aureus.
Grigg et al. (2010)
3 more