› 3bed

X-ray diffraction
1.45Å resolution

Mannose/sorbose specific IIA subunit of phosphotransferase system from Enterococcus faecalis

Released:
DOI: 10.2210/pdb3bed/pdb
PDB entry 3bed coloured by chain, front view.
PDB entry 3bed coloured by chain, side view.
PDB entry 3bed coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Osipiuk J, Wu R, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-182872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS EIIA type-4 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 15.3 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q838I6 (Residues: 1-139; Coverage: 100%)
Gene name: EF_0461
Sequence domains: PTS system fructose IIA component
Structure domains: Phosphotransferase system, mannose-type IIA component

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 18 x MSE
Ligand MLY 4 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 106.059Å b: 37.503Å c: 76.993Å
α: 90° β: 121.49° γ: 90°
R-values:
R R work R free
0.159 0.157 0.197
Expression system: Escherichia coli BL21(DE3)

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