› 3beu

X-ray diffraction
1.05Å resolution

Na+-Dependent Allostery Mediates Coagulation Factor Protease Active Site Selectivity

Released:
DOI: 10.2210/pdb3beu/pdb
Model geometry
Fit model/data
PDB entry 3beu coloured by chain, front view.
PDB entry 3beu coloured by chain, side view.
PDB entry 3beu coloured by chain, top view.
Source organism: Streptomyces griseus
Primary publication:
Engineering protein allostery: 1.05 A resolution structure and enzymatic properties of a Na+-activated trypsin.
Page MJ, Carrell CJ, Di Cera E
J Mol Biol 378 666-72 (2008)
PMID: 18377928

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133480 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trypsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 23.54 KDa
Source organism: Streptomyces griseus
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P00775 (Residues: 37-115, 116-259; Coverage: 98%)
Gene name: sprT
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

4 bound ligands:
Ligand SO4 11 x SO4
Ligand BEN 4 x BEN
Ligand NA 2 x NA
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 41.786Å b: 72.433Å c: 122.492Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.121 0.12 0.146
Expression system: Bacillus subtilis

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Citations

5 review citations

Biology of human sodium glucose transporters.
Wright et al. (2011)
4 more

1 mention without citation

Engineering protein allostery: 1.05 A resolution structure and enzymatic properties of a Na+-activated trypsin.
Page et al. (2008)