› 3bew

X-ray diffraction
2.6Å resolution

10mer Crystal Structure of chicken MHC class I haplotype B21

Released:
DOI: 10.2210/pdb3bew/pdb
PDB entry 3bew coloured by chain, front view.
PDB entry 3bew coloured by chain, side view.
PDB entry 3bew coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structures of an MHC class I molecule from B21 chickens illustrate promiscuous peptide binding.
Koch M, Camp S, Collen T, Avila D, Salomonsen J, Wallny HJ, van Hateren A, Hunt L, Jacob JP, Johnston F, Marston DA, Shaw I, Dunbar PR, Cerundolo V, Jones EY, Kaufman J
Immunity 27 885-99 (2007)
PMID: 18083574

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-140589 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 271 amino acids
Theoretical weight: 30.93 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q95601 (Residues: 22-291; Coverage: 81%)
Gene names: B-FIV, BF2, BFIV21
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.19 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21611 (Residues: 22-119; Coverage: 100%)
Gene name: B2M
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Tubulin beta-6 chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 10 amino acids
Theoretical weight: 1.19 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P09207 (Residues: 324-333; Coverage: 2%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 57.393Å b: 88.711Å c: 100.23Å
α: 90° β: 80.01° γ: 90°
R-values:
R R work R free
0.237 0.234 0.295
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

16 review citations

The impact of host genetic variation on infection with HIV-1.
McLaren et al. (2015)
15 more

11 mentions without citation

New vistas unfold: Chicken MHC molecules reveal unexpected ways to present peptides to the immune system.
Halabi et al. (2022)
10 more