› 3bga

X-ray diffraction
2.1Å resolution

Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482

Released:
DOI: 10.2210/pdb3bga/pdb
PDB entry 3bga coloured by chain, front view.
PDB entry 3bga coloured by chain, side view.
PDB entry 3bga coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Entry authors: Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183733 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 1010 amino acids
Theoretical weight: 116.19 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8A799 (Residues: 24-1022; Coverage: 100%)
Gene name: BT_1626
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand NA 4 x NA
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 42 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 88.335Å b: 138.864Å c: 102.818Å
α: 90° β: 109.73° γ: 90°
R-values:
R R work R free
0.2 0.2 0.234
Expression system: Escherichia coli BL21(DE3)

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