› 3bh0

X-ray diffraction
2.35Å resolution

ATPase Domain of G40P

Released:
DOI: 10.2210/pdb3bh0/pdb
PDB entry 3bh0 coloured by chain, front view.
PDB entry 3bh0 coloured by chain, side view.
PDB entry 3bh0 coloured by chain, top view.
Source organism: Bacillus phage SPP1
Primary publication:
The structure of a DnaB-family replicative helicase and its interactions with primase.
Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS
Nat Struct Mol Biol 15 94-100 (2008)
PMID: 18157148

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA helicase Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 35.52 KDa
Source organism: Bacillus phage SPP1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38152 (Residues: 130-442; Coverage: 71%)
Sequence domains: DnaB-like helicase C terminal domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P61
Unit cell:
a: 96.779Å b: 96.779Å c: 54.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.238 0.285
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
11 more

4 mentions without citation

The bacterial DnaC helicase loader is a DnaB ring breaker.
Arias-Palomo et al. (2013)
3 more