› 3bhg

X-ray diffraction
1.9Å resolution

Crystal structure of adenylosuccinate lyase from Legionella pneumophila

Released:
DOI: 10.2210/pdb3bhg/pdb
PDB entry 3bhg coloured by chain, front view.
PDB entry 3bhg coloured by chain, side view.
PDB entry 3bhg coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Entry authors: Chang C, Li H, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chain: A
Molecule details ›
Chain: A
Length: 459 amino acids
Theoretical weight: 52.29 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZXD1 (Residues: 1-456; Coverage: 100%)
Gene names: lpg0801, purB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4122
Unit cell:
a: 109.216Å b: 109.216Å c: 157.099Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.195
Expression system: Escherichia coli

Quick links