› 3bhs

X-ray diffraction
1.99Å resolution

Nitrosomonas europaea Rh50 and mechanism of conduction by Rhesus protein family of channels

Released:
DOI: 10.2210/pdb3bhs/pdb
PDB entry 3bhs coloured by chain, front view.
PDB entry 3bhs coloured by chain, side view.
PDB entry 3bhs coloured by chain, top view.
Source organism: Nitrosomonas europaea ATCC 19718
Entry authors: Gruswitz F, Ho C-M, del Rosario MC, Westhoff CM, Stroud RM, Center for Structures of Membrane Proteins (CSMP)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter AmtB-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 42.52 KDa
Source organism: Nitrosomonas europaea ATCC 19718
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82X47 (Residues: 26-425; Coverage: 100%)
Gene names: NE0448, Rh50
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: R3
Unit cell:
a: 96.675Å b: 96.675Å c: 137.621Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.237
Expression system: Escherichia coli BL21(DE3)

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