› 3bif

X-ray diffraction
2.3Å resolution

6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE EMPTY 6-PF-2K ACTIVE SITE

Released:
DOI: 10.2210/pdb3bif/pdb
PDB entry 3bif coloured by chain, front view.
PDB entry 3bif coloured by chain, side view.
PDB entry 3bif coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A switch in the kinase domain of rat testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
Yuen MH, Wang XL, Mizuguchi H, Uyeda K, Hasemann CA
Biochemistry 38 12333-42 (1999)
PMID: 10493801

Function and Biology Details

Reactions catalysed: 
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate
Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150388 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 Chain: A
Molecule details ›
Chain: A
Length: 468 amino acids
Theoretical weight: 53.94 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P25114 (Residues: 2-469; Coverage: 100%)
Gene name: Pfkfb4
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand BOG 2 x BOG
Ligand PO4 2 x PO4
Ligand SIN 1 x SIN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 106.7Å b: 108.7Å c: 71.7Å
α: 90° β: 129.5° γ: 90°
R-values:
R R work R free
0.197 0.197 0.243
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Role of PFKFB3 and PFKFB4 in Cancer: Genetic Basis, Impact on Disease Development/Progression, and Potential as Therapeutic Targets.
Kotowski et al. (2021)
1 more