› 3big

X-ray diffraction
1.85Å resolution

Crystal structure of the fructose-1,6-bisphosphatase GlpX from E.coli in complex with inorganic phosphate

Released:
DOI: 10.2210/pdb3big/pdb
PDB entry 3big coloured by chain, front view.
PDB entry 3big coloured by chain, side view.
PDB entry 3big coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural and biochemical characterization of the type II fructose-1,6-bisphosphatase GlpX from Escherichia coli.
Brown G, Singer A, Lunin VV, Proudfoot M, Skarina T, Flick R, Kochinyan S, Sanishvili R, Joachimiak A, Edwards AM, Savchenko A, Yakunin AF
J Biol Chem 284 3784-92 (2009)
PMID: 19073594

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141867 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase 1 class 2 Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 36.05 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9C9 (Residues: 1-336; Coverage: 100%)
Gene names: JW3896, b3925, glpX
Sequence domains: Bacterial fructose-1,6-bisphosphatase, glpX-encoded
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand UNX 1 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P422
Unit cell:
a: 91.348Å b: 91.348Å c: 86.366Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.239
Expression system: Escherichia coli

Quick links

Citations

3 review citations

High-throughput crystallography for structural genomics.
Joachimiak A. (2009)
2 more

5 mentions without citation

Structural and biochemical characterization of the type II fructose-1,6-bisphosphatase GlpX from Escherichia coli.
Brown et al. (2009)
4 more