› 3bj9

X-ray diffraction
2Å resolution

Crystal structure of the Surrogate Light Chain Variable Domain VpreBJ

Released:
DOI: 10.2210/pdb3bj9/pdb
PDB entry 3bj9 coloured by chain, front view.
PDB entry 3bj9 coloured by chain, side view.
PDB entry 3bj9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering and characterization of a single chain surrogate light chain variable domain.
Morstadt L, Bohm A, Yüksel D, Kumar K, Stollar BD, Baleja JD
Protein Sci 17 458-65 (2008)
PMID: 18287279

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin iota chain Chain: 1
Molecule details ›
Chain: 1
Length: 116 amino acids
Theoretical weight: 12.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12018 (Residues: 22-122; Coverage: 80%)
Gene names: VPREB, VPREB1
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup: I222
Unit cell:
a: 43.811Å b: 67.176Å c: 99.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.23
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

In vivo imaging of prostate cancer using an anti-PSMA scFv fragment as a probe.
Mazzocco et al. (2016)
3 more

3 mentions without citation

Antibody CDR loops as ensembles in solution vs. canonical clusters from X-ray structures.
Fernández-Quintero et al. (2020)
2 more